Liberation of Zinc-Containing L31 (RpmE) from Ribosomes by Its Paralogous Gene Product, YtiA, in Bacillus subtilis
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چکیده
منابع مشابه
The mechanism of liberation of penicillinase from Bacillus subtilis.
The effects of various factors on the release of penicillinase from apparently undamaged cells of a growing culture of Bacillus subtilis were investigated. The enzyme was not eluted from the cells by treating them with high concentrations of salt. Its liberation did not take place at all a t Oo, and was nearly completely inhibited a t pH values below 6.0, whereas chloramphenicol, at concentrati...
متن کاملProperties of 5-fluorouracil-containing ribonucleic acid and ribosomes from Bacillus subtilis.
Growth of a strain of Bacillus subtilis that requires uracil, thymine, adenine, and tryptophan in the presence of 5-fluorouracil (FU) results in the synthesis of ribonucleic acid (RNA) and ribosomes in which 55 to 65% of the RNA uracil has been replaced by the fluorine derivative. Examination of analogue-containing ribosomes by sucrose density gradient centrifugation and thermal denaturation st...
متن کاملFunctional analysis of paralogous thiol-disulfide oxidoreductases in Bacillus subtilis.
The in vivo formation of disulfide bonds, which is critical for the stability and/or activity of many proteins, is catalyzed by thiol-disulfide oxidoreductases. In the present studies, we show that the Gram-positive eubacterium Bacillus subtilis contains three genes, denoted bdbA, bdbB, and bdbC, for thiol-disulfide oxidoreductases. Escherichia coli alkaline phosphatase, containing two disulfid...
متن کاملMolecular Detection of Lipase A gene in Putative Bacillus subtilis Strains Isolated from Soil
The present study was undertaken to screen the soil samples collected in Iran for the presence of the Bacillus subtilis lipase A gene. The bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and the polymerase chain reaction (PCR). Only four colonies were identified as putative B. subtilis strains and all contained the lipase A ge...
متن کاملRecombination between compatible plasmids containing homologous segments requires the Bacillus subtilis recE gene product.
Plasmid pSL103 was previously constructed by cloning a Trp fragment (approximately 2.3 X 10(6) daltons) from restriction endonuclease EcoRI-digested chromosome DNA of Bacillus pumilus using the neomycin-resistance plasmid pUB110 (approximately 2.8 X 10(6) daltons) as vector and B. subtilis as transformation recipient. In the present study the EcoRI Trp fragment from pSL103 was transferred in vi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2006
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.188.7.2715-2720.2006